Fluorescence-detected linear dichroism imaging in a re-scan confocal microscope equipped with differential polarization attachment
نویسندگان
چکیده
منابع مشابه
Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics
Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumina...
متن کاملHigh frame-rate fluorescence confocal angle-resolved linear dichroism microscopy.
Angle-resolved linear dichroism is a recent technique that exploits images recorded using an illumination field whose polarization angle is sequentially rotated during acquisition. It allows to retrieve orientation information of the fluorescent molecules, namely the average orientation angle and the amplitude of the fluctuations around this average. In order to boost up the acquisition speed w...
متن کاملVideo-rate in vivo fluorescence imaging with a line-scanned dual-axis confocal microscope.
Video-rate optical-sectioning microscopy of living organisms would allow for the investigation of dynamic biological processes and would also reduce motion artifacts, especially for in vivo imaging applications. Previous feasibility studies, with a slow stage-scanned line-scanned dual-axis confocal (LS-DAC) microscope, have demonstrated that LS-DAC microscopy is capable of imaging tissues with ...
متن کاملConfocal fluorescence microscopy with the tandem scanning light microscope.
Applications of the tandem scanning confocal microscope (TSM) to fluorescence microscopy and its ability to resolve fluorescent biological structures are described. The TSM, in conjunction with a cooled charge-coupled device (cooled CCD) and conventional epifluorescence light source and filter sets, provided high-resolution, confocal data, so that different fluorescent cellular components were ...
متن کاملImaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope.
Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8,333 lines/sec b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Biophysics Journal
سال: 2019
ISSN: 0175-7571,1432-1017
DOI: 10.1007/s00249-019-01365-4